Light Microscopy Resource Hub
Molecular Expressions Microscopy Primer
This page is an educational online resource that explains the fundamentals of optical microscopy, including light physics, microscope components, imaging techniques, and digital image processing. It also offers tutorials and interactive tools for students and researchers.
Evident Microscopy Resource Center
A comprehensive online microscopy resource providing educational content on optical principles, imaging techniques, fluorescent proteins, and microscope fundamentals for researchers and students.
This page provides a clear introduction to fluorescence microscopy, explaining the basic principles of fluorescence, how excitation and emission light are separated using optical filters, and how fluorescence signals are detected. It also covers key concepts such as fluorophores, Stokes shift, photobleaching, and the main components of fluorescence microscope systems.
Thermo Fisher Fluorescence Fundamentals
Provides a clear overview of the fundamental principles of fluorescence, including how fluorescent probes work, how signals are generated and detected, and how fluorescence is applied in biological and biochemical research. It also explains key concepts such as excitation, emission, spectra, and instrumentation used in fluorescence-based experiments.
Fiji (ImageJ distribution) is a free, open-source platform for scientific image processing and analysis, bundling ImageJ with a wide range of plugins for tasks like visualization, segmentation, and automation.
Free, open-source tool that enables researchers to automatically analyze and quantify biological images, using customizable pipelines to measure cellular features without requiring programming skills.
Open-source platform for analyzing whole-slide and microscopy images, widely used for digital pathology and quantitative image analysis.
Open-source Python-based platform for interactive visualization, annotation, and analysis of large multi-dimensional images, widely used in scientific imaging workflows.
User-friendly, open-source tool for interactive image analysis that uses machine learning to help with segmentation, classification, counting, and tracking in microscopy images. It lets users train models simply by labeling examples, without requiring programming or ML expertise.
FPbase is a free, open-source database of fluorescent proteins, offering structured, searchable information such as sequences, properties, structural data, and key literature for the imaging community.
Thermo Fisher Fluorophore Selection Guide
Provides a comprehensive guide to fluorescent dyes (fluorophores) used in cell analysis, helping researchers select and compare fluorophores based on their spectral properties, applications, and compatibility for microscopy and flow cytometry experiments.
Chroma Glossary of Optical Terms
This page is a comprehensive glossary of optical and fluorescence microscopy terms, providing clear definitions of key concepts related to optical filters, light behavior, and imaging technologies.